Agilent Screentape Assays: High Sensitivity DNA Analysis & More
In the rapidly evolving landscape of molecular biology, where precision and efficiency are paramount, how can researchers ensure the accurate and rapid analysis of precious DNA samples? The Agilent D5000 and High Sensitivity D5000 Screentape Assays, coupled with the Agilent 4200 TapeStation system, provide a robust and streamlined solution, offering unparalleled speed and accuracy for DNA fragment analysis.
These assays, designed for the separation and analysis of DNA fragments ranging from 100 base pairs (bp) to 5,000 bp, have become indispensable tools for a wide array of applications, from library preparation for Next-Generation Sequencing (NGS) to quality control of PCR and qPCR products. Their ability to analyze precious samples with minimal input and deliver objective, quantitative data makes them a cornerstone for modern molecular biology workflows.
| Feature | Description | Benefit |
|---|---|---|
| Assay Types | D1000, High Sensitivity D1000, D5000, High Sensitivity D5000, RNA Screentape, High Sensitivity RNA Screentape | Versatile applications for DNA and RNA analysis |
| Fragment Size Range | 100 bp to 5,000 bp | Suitable for a broad range of DNA and RNA fragment sizes |
| Sample Throughput | Up to 96 samples per run (with the Agilent 4200 TapeStation system) | High-throughput analysis capabilities |
| Analysis Time | 1 to 2 minutes per sample | Fast analysis times, increasing lab efficiency |
| Sample Input | As little as 2 l of DNA sample required | Conserves precious samples |
| Reagents | Sample buffer, ladder | High-quality reagents ensure accurate results |
| Applications | Library QC for NGS, PCR and qPCR product QC | Essential for molecular biology workflows |
The Agilent technologies high sensitivity d5000 reagent helps in the high sensitive analysis of dna in the range of 100 to 5000 bp. This reagent comes with a ladder and buffer samples that are efficient to test up to 105 samples. For the high sensitivity analysis of dna from 100 to 5000 bp. For the high sensitivity analysis of dna from 100 to 5000 bp. Retrieve the screentape, sample buffer, and ladder (if necessary) for your chosen assay at least 30 minutes
The Agilent D5000 and High Sensitivity D5000 Screentape Assays are not merely tools; they are transformative technologies, enabling researchers to unlock deeper insights with unprecedented speed and accuracy. Their ease of use, coupled with their ability to handle precious samples with minimal input, makes them an essential asset in any modern molecular biology laboratory. Whether it's library preparation for NGS, quality control of PCR products, or the analysis of fragmented DNA, these assays consistently deliver reliable and reproducible results.
The core of the system is the Agilent 4200 TapeStation system. This automated platform provides a streamlined workflow for DNA and RNA electrophoresis. The system utilizes pre-packaged reagents and minimizes manual handling, allowing for up to 96 samples to be processed in a single run. This automated approach not only saves time but also reduces the potential for human error, ensuring consistent and reliable results.
The high sensitivity DNA screentape assays are particularly well-suited for the analysis of precious samples. With only 2 l of sample needed for analysis, researchers can minimize sample consumption, conserving valuable material for downstream experiments. The assays can separate DNA fragments and libraries up to 5000 base pairs in just 1 to 2 minutes per sample. This speed and efficiency are crucial in high-throughput environments where time is of the essence.
The high sensitivity D5000 reagents, including the sample buffer and ladder, are designed to provide optimal performance. For 105 samples, the catalog code is provided, and the preparation protocol is straightforward: For each sample, 10 l of D5000 sample buffer and 1 l of D5000 ladder are pipetted into a tube strip at position A1. For larger sample batches (16 or more), the protocol is adjusted to 20 l of sample buffer and 2 l of ladder, ensuring that the assay remains sensitive and accurate regardless of the number of samples.
The Agilent D1000 screentape assay (a) and high sensitivity D1000 screentape assay (b) offer sample preparation protocols that are easy to follow, guaranteeing that even the most complex molecular biology workflows are simplified. The high sensitivity assays, in particular, are designed to deliver high-quality data with minimal sample input, enabling detailed analysis even when dealing with limited material.
The system's ability to provide objective, quantitative, and purity measurements from a minimal sample volume makes it an invaluable asset. The data output provides essential information about the size distribution, concentration, and integrity of the DNA or RNA fragments, enabling researchers to make informed decisions about the suitability of their samples for downstream applications.
The Agilent high sensitivity D1000 and high sensitivity D5000 screentape assays are particularly well-suited for low-abundance and precious DNA samples. When combined with the TapeStation instrument, these assays provide a rapid, automated, and straightforward solution for library quality control (QC) in next-generation sequencing (NGS) workflows, as well as for PCR and qPCR product QC. The speed and ease of use of these assays make them essential in fast-paced research and diagnostic environments.
The screentape devices themselves are designed for ease of use and efficiency. Each device includes a screentape, sufficient for 105 samples. This thoughtful design ensures that researchers have the necessary tools for high-throughput analysis readily available.
The assays are developed for the separation and analysis of DNA fragments from 100 bp to 5,000 bp, a size range that complements the needs of various molecular biology applications. Fragments of 500 bp and 3,000 bp in length can be prepared for accurate analysis.
For the high sensitivity analysis of DNA from 100 to 5000 bp, the high sensitivity d5000 screentape assays are an ideal choice. In recent years, the next-generation sequencer (NGS) has been used in experiments, and in order to obtain high-quality sequencing data, it is necessary to perform the fragmentation of the extracted DNA and the sequencing library.
In addition to the technical specifications, the use of these assays also comes with safety considerations. The article highlights that when used under reasonable conditions and in accordance with the directions for use, the product should not present a health hazard. Warning about toxic agents is also included, advising users to refer to the product material safety datasheets for further information, ensuring that researchers are well-informed about the safe handling of the reagents.
The Agilent D5000 and High Sensitivity D5000 Screentape Assays are available from Agilent Technologies. The supplier information provides details such as the catalog code for the reagents and devices, ensuring that researchers can easily procure the necessary materials for their experiments.
The combination of automated electrophoresis with pre-packaged reagents streamlines the workflow and reduces the possibility of human errors, increasing the reproducibility and reliability of the results.
